The Abscisic Acid Content of Citrus with Special Reference to Lemon

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Abstract
Free and conjugated abscisic acid (ABA) in the fruit peel of four species of citrus and their distribution in the various reproductive organs of Citrus limon L. cv. Eureka during ontogeny were determined by an indirect enzyme-linked immunosorbent assay (ELISA). The different citrus species varied in free ABA (3-8 g g dry wt.) and conjugated ABA (10-39 /xg gl dry wt.). In general, the amount of conjugated ABA was 4-fold that of free ABA. In the lemon flower, the combined style and stigma tissues contained the predominant (>65%) amount of both free and conjugated ABA. During fruit development, free and conjugated ABA of the different tissues showed dynamic changes. In the vesicles, free ABA showed progressive increases with development and reached a high level at maturity whereas the conjugated ABA showed a corresponding decrease with fruit development. Free ABA in the seed increased with fruit development. The content of ABA in citrus fruit was significantly correlated (ŷ = 8.06 + 2.81 In x; /-2 = 0.84**) with the weight of the fruit

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