Preparation of a stable non-infective western equine encephalomyelitis virus antigen from allantoic fluid

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Abstract
It was demonstrated that a potent and specific non-infective diagnostic western equine encephalomyelitis virus antigen can be readily prepared in quantity by gamma irradiation of the infective allantoic fluid, followed by its concentration and lyophilization for stable storage. This antigen is easier to prepare in bulk and involves less exposure of personnel to the infective agent than the antigen prepared from suckling mouse brains.