Anatomy of herpes simplex virus DNA. VI. Defective DNA originates from the S component
- 1 November 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 20 (2) , 527-531
- https://doi.org/10.1128/jvi.20.2.527-531.1976
Abstract
We previously reported that serial propagation of the Justin strain of herpes simplex virus 1 [HSV-1 (Justin)] results in the generation of defective DNA molecules consisting of tandem repetitions of sequences of limited complexity. In the present study, HSV-1 DNA was cleaved with the restriction endonucleases BglII and EcoRI. The fragments were electrophoretically separated on agarose gels, transferred to nitrocellulose strips, and then hybridized with 32P-labeled HSV-1 (Justin) defective DNA. The data allow us to conclude that DNA sequences contained in the repeat unit of defective DNA originate from the S segment of the wild-type viral DNA molecule.This publication has 18 references indexed in Scilit:
- Detection of specific sequences among DNA fragments separated by gel electrophoresisPublished by Elsevier ,2006
- Anatomy of herpes simplex virus DNA: evidence for four populations of molecules that differ in the relative orientations of their long and short components.Proceedings of the National Academy of Sciences, 1975
- Anatomy of herpes simplex virus DNA: strain differences and heterogeneity in the locations of restriction endonuclease cleavage sites.Proceedings of the National Academy of Sciences, 1975
- Cyclic Appearance of Defective Interfering Particles of Herpes Simplex Virus and the Concomitant Accumulation of Early Polypeptide VP175Intervirology, 1975
- Regulation of Herpesvirus Macromolecular Synthesis: Nuclear Retention of Nontranslated Viral RNA SequencesProceedings of the National Academy of Sciences, 1974
- Analysis of DNA of Defective Herpes Simplex Virus Type 1 by Restriction Endonuclease Cleavage and Nucleic Acid HybridizationCold Spring Harbor Symposia on Quantitative Biology, 1974
- Inverted Repetitions in the Chromosome of Herpes Simplex VirusCold Spring Harbor Symposia on Quantitative Biology, 1974
- The evolution of new species of viral DNA during serial passage of simian virus 40 at high multiplicityVirology, 1973
- Defective Virions of Herpes Simplex VirusesIntervirology, 1973
- Herpes simplex virus DNAVirology, 1968