ANTIBODY ACTIVITY IN STICK-ELISA AS COMPARED TO OTHER QUANTITATIVE IMMUNOLOGICAL TESTS IN SERA OF ECHINOCOCCOSIS CASES

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  • 1 January 1978
    • journal article
    • research article
    • Vol. 29  (4) , 417-422
Abstract
Antibody [Ab] activity in sera of 43 [human] cases with cystic and 5 cases with alveolar echinococcosis was determined against hydatid fluid and extrace of Echinococcus alveolaris. The results with stick-ELISA [enzyme-linked immunosorbent assay] were compared to those obtained with complement fixation [CF] and indirect hemagglutination [HA]. Results in ELISA were determined in stepless fashion and expressed as a multiple of normal activity (MONA). Sensitivities of the different tests with reference to their respective ranges of nonrelevant activities were 95% in ELISA, 95% in indirect HA and 90% in CF as determined with sera from cystic echinococcosis cases and their homologous antigen [Ag]. Employing homologous and heterologous Ag, of the 3 immunotests allowed the species specific diagnosis of cystic echinococcosis cases, whereas most cases of alveolar echinococcosis could be recognized by their predominant homologous Ab activity in ELISA and CF.