Determination of Vitamin K in Serum Using HPLC with Post-Column Reaction and Fluorescence Detection
- 1 February 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 24 (2) , 76-79
- https://doi.org/10.1093/chromsci/24.2.76
Abstract
A new, sensitive and selective detection method for vitamin K1 in human serum is reported. After the chromatographic separation, vitamin K is converted to its hydro-quinone form in a wet-chemical post-column reduction reaction with tetramethylammonium octahydridotriborate. The reaction proceeds in an open tubular knitted reaction coil at elevated temperature. This system, when combined with a two-step multidimensional liquid chromatographic separation, has proven useful for the quantitative determination of serum concentrations of vitamin K1 in normal, healthy individuals.This publication has 5 references indexed in Scilit:
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