Regulation of calcium sensitivity in perforated mammalian cardiac cells.
Open Access
- 1 February 1983
- journal article
- research article
- Published by Rockefeller University Press in The Journal of general physiology
- Vol. 81 (2) , 195-211
- https://doi.org/10.1085/jgp.81.2.195
Abstract
Sarcolemmal perforations can be produced in bundles of rat right ventricular cells by either perfusion of the heart or soaking of the bundles with a solution containing 10 mM EGTA. All cells are affected and lose approximately 40% of the surface membrane. In these cells it is possible to show cAMP regulation of contractility (maximum Ca-activated force) without cAMP regulation of Ca sensitivity (pCa for 50% of maximum Ca-activated force). Therefore, the target molecule for cAMP is different for the two regulatory systems. Both regulatory systems can be slowly washed out of the cell by 10 mM EGTA solution but not by relaxing or contraction solutions. A model for regulation of Ca sensitivity is proposed.This publication has 20 references indexed in Scilit:
- Isolation of cardiac myofibrils and myosin light chains with in vivo levels of light chain phosphorylationBiochimica et Biophysica Acta (BBA) - General Subjects, 1979
- Phosphorylation and dephosphorylation of phosphorylase kinase in the perfused rat heart.Journal of Biological Chemistry, 1979
- PROSTAGLANDIN-E1 ACTIVATION OF HEART CAMP-DEPENDENT PROTEIN-KINASE - APPARENT DISSOCIATION OF PROTEIN-KINASE ACTIVATION FROM INCREASES IN PHOSPHORYLASE-ACTIVITY AND CONTRACTILE-FORCE1979
- The regulation of the calcium sensitivity of the contractile system in mammalian cardiac muscle.The Journal of general physiology, 1978
- Compartmentalization of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in heart tissue.Journal of Biological Chemistry, 1977
- Studies on the phosphorylation of the inhibitory subunit of troponin during modification of contraction in perfused rat heartBiochemical Journal, 1976
- Phosphorylation of the inhibitory subunit of troponin and its effect on the calcium dependence of cardiac myofibril adenosine triphosphataseFEBS Letters, 1976
- Contractions induced by a calcium‐triggered release of calcium from the sarcoplasmic reticulum of single skinned cardiac cells.The Journal of Physiology, 1975
- Correlation between contraction and phosphorylation of the inhibitory subunit of troponin in perfused rat heartFEBS Letters, 1975
- The Reliability of Molecular Weight Determinations by Dodecyl Sulfate-Polyacrylamide Gel ElectrophoresisJournal of Biological Chemistry, 1969