RAPID DIAGNOSTIC DNA CYTOMETRY WITH AN AUTOMATIC MICROSCOPE AND A TV IMAGE-ANALYSIS SYSTEM

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  • 1 January 1984
    • journal article
    • research article
    • Vol. 6  (3) , 179-188
Abstract
A television-based image-analysis system (Leitz TAS plus) combined with a Leitz automatic microscope was used for rapid DNA cytometry for diagnostic purposes. Malignant suspicious cells found by the cytopathologists in conventionally stained smears were automatically relocated and measured. A program for automatic detection and measurement of nuclear DNA content was developed. DNA data are processed by an algorithm for the diagnosis and grading of malignancy. This diagnostic procedure is performed in real time and yields highly reproducible results. With this equipment, diagnostic DNA cytometry may be introduced in the routine cytology laboratory. Our mode of application does not replace the cytopathologists, but assists him or her in cases of doubtful diagnosis and in the grading of malignant tumors.

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