The rapid purification of 3-hydroxybutyrate dehydrogenase and malate dehydrogenase on triazine dye affinity matrices

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Abstract
3-Hydroxybutyrate dehydrogenase (EC 1.1.1.30) and malate dehydrogenase (EC 1.1.1.37) were purified to homogeneity [from Rhosopseudomonas sphaeroides] on a large scale involving only 2 sequential affinity chromatography steps on 2 triazine dye-Sepharose matrices. Recoveries of both enzymes were in excess of 60%. Malalte dehydrogenase could also be purified by a combination of triazine dye affinity chromatography and gel filtration on Ultrogel AcA-44, but this offered no significant advantage over the purely affinity procedure.