Imaging of Vapor Plumes Produced by Matrix Assisted Laser Desorption: A Plume Sharpening Effect
- 12 July 1999
- journal article
- research article
- Published by American Physical Society (APS) in Physical Review Letters
- Vol. 83 (2) , 444-447
- https://doi.org/10.1103/physrevlett.83.444
Abstract
The first gated imaging of both light matrix molecule and heavy biomolecule vapor expansion during matrix assisted laser desorption and ionization is reported, revealing a plume sharpening effect. Laser induced fluorescence imaging of dye-tagged DNase I proteins shows that these heavy molecules (30 000 Da) propagate within a very narrow angular distribution compared to that of the 3-HPA matrix (139 Da). A special solution of the gas dynamic equations is developed to describe the 3D coexpansion of heavy and light plume components.Keywords
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