A "Mix and Read" Assay for Insulin Using Fluorometric Microvolume Assay Technology
- 1 August 2003
- journal article
- research article
- Published by Mary Ann Liebert Inc in ASSAY and Drug Development Technologies
- Vol. 1 (4) , 521-525
- https://doi.org/10.1089/154065803322302772
Abstract
We describe a novel, "mix and read" immunoassay for insulin in biological samples using FMAT. Current commercial assays for insulin require multiple washing steps and can be expensive. The insulin assay described is a simple two-step, time-saving assay and amenable to robotics. The linear response for the fluorometric signal is comparable to that observed using classical ELISA and RIA. A series of mouse plasma samples were tested for insulin levels and yielded results comparable to that measured using a commercial ELISA for insulin.Keywords
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