Solid-Phase Radioimmunoassay for Detecting Bovine (Neonatal Calf Diarrhea) Rotavirus Antibody

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Abstract
An indirect solid-phase microradioimmunoassay is described for detecting antibodies against rotaviruses. The test involved ethanol fixation of microcultures of bovine rotavirus-infected [African green monkey kidney] BSC-1 cells and reaction with bovine anti-rotavirus serum, followed by 125I-labeled rabbit anti-bovine immunoglobulin G. The technique was virus specific and highly sensitive. The fixed microcultures could be stored at 4.degree. C for at least 2 mo. without affecting the sensitivity of the test. The application of this system for the detection of rotavirus antibodies in humans is briefly discussed.