Abstract
A new species of Pseudomonas was isolated from soil by the enrichment culture technique with citronellol as the sole carbon source. The morphological and nutritional characteristics of the organism are described, and the name Pseudomonas citronellolis n. sp. is proposed. Dried cell preparations catalyze the oxidation of the isoprenoids farnesol and citronellol, as well as a number or related compounds which could be intermediates in isoprenoid degradation in this organism. Isotope studies on the metabolism of acetate in dried cells indicate that this substrate, a likely intermediate in isoprenoid degradation, is converted partially to ether-soluble fluorescent pigments as well as to protein amino acids. The purification of the pigments is described. Using chromatographic procedures it was shown that tyrosine, phenylalanine, and leucine were preferentially synthesized from acetate. A method for the quantitative determination of citronellol and of citronellic acid is described.
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