A specific enzyme electrode for spermine and spermidine
- 1 January 1983
- journal article
- Published by Institute of Organic Chemistry & Biochemistry in Collection of Czechoslovak Chemical Communications
- Vol. 48 (2) , 672-678
- https://doi.org/10.1135/cccc19830672
Abstract
A sensor method has been worked out for the determination of spermine and spermidine in concentrations 0.02-0.5 mmol l-1. The method is based on amperometric measurement of the consumption of oxygen and the formation of hydrogen peroxide in a reaction catalyzed by crosslinked polyamine oxidase from maize. One determination takes 1 to 2 min, with phosphate at least 8 months. The optimum conditions for the analysis are described and the applicability of the sensor to urine samples is dealt with.Keywords
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