Quantitative changes in the catalytic and regulatory subunits of nuclear cAMP‐dependent protein kinases type I and type II during isoproterenol‐induced growth of the rat parotid gland

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Abstract
To quantify the cAMP‐dependent protein kinases I and II in parotid gland nuclei independent of the enzyme activity, monospecific antisera against their subunits were applied in a sensitive enzyme immunoassay. About 3% of total catalytic subunit in the homogenate was found in the isolated nuclei. During β‐agonistinduced proliferation of the parotid gland the nuclear concentration of catalytic and regulatory subunits changed. Related to the number of nuclei, the catalytic subunit and the regulatory subunit RI increased about 3‐fold whereas the regulatory subunit RII remained unchanged.