Rejuvenation and Freezing of Outdated Stored Human Red Cells

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Abstract
Red cells stored in acid citrate dextrose for about 23 days, incubated with pyruvate, inosine, glucose, and phosphate for 60 minutes at 37°C, frozen with 40 per cent wt/vol of glycerol, stored at -80°C for 12 months, and then thawed and washed, had normal oxygen-transport function and 24-hour post-transfusion survivals of about 75 per cent. After red cells had been stored in acid citrate dextrose at 4°C for about 29 days, incubated with pyruvate, inosine, glucose, phosphate, and adenine, glycerolized, frozen, thawed, and washed, they had normal oxygen-transport function and 24-hour post-transfusion survivals of about 80 per cent. About 90 per cent of the red cells were recovered after rejuvenation, freezing, thawing, and washing.