Identification and characterization of double-stranded RNA associated with cytoplasmic male sterility in Vicia faba
- 1 November 1981
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 78 (11) , 7043-7046
- https://doi.org/10.1073/pnas.78.11.7043
Abstract
The cytoplasmic male sterility (CMS) trait of at least 1 line of V. faba plants is always associated with the presence of high MW double-stranded RNA in the leaf tissue extracts. Subcellular fractions of leaf tissue from CMS and fertile maintainer plants were initially analyzed in an attempt to locate, identify and characterize the genetic material involved with the sterility trait. This CMS-associated high MW RNA was found only in the cytosol of the 447 male sterile line of V. faba plants and could not be isolated from the recurrent parent (maintainer), from lines that had been fertility-restored, or from lines that had reverted from the sterile condition. CMS-associated RNA was moved from donor to fertile host plants through a dodder [Cuscuta subinclusa] bridge. These hosts not only contain the RNA but now exhibit a male sterile phenotype, as detected by visual examination of the flower, the pollen, morphological characteristics and pollen staining ability.This publication has 19 references indexed in Scilit:
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