Biochemical Research on Oogenesis

Publisher Website
Google Scholar
Abstract
The properties of tRNA from oocytes of Xenopus laevis were compared with those of tRNA from somatic cells of the same species. Both types of tRNAs were found to have the same average length and to contain an equal variety of modified nucleotides. However, tRNA from small oocytes differed from somatic tRNA by its chromatographic behavior on methylated-albumin kieselguhr columns. The elution profiles from reversed-phase chromatography 5 columns of several aminoacyl tRNAs were compared after charging somatic and oocyte tRNSs with 3H or 14C-labelled amino acids. Striking differences in peak position were observed when tRNA from small oocytes was mixed and co-chromatographed with somatic tRNA. The differences were less important when tRNAs from large oocytes and from somatic cells were compared. Mixtures of egg and somatic tRNAs gave completely or almost completely coincident elution profiles. Only one isoacceptor, tNRA1Met (initiator tRNA) had the same position in all reversed-phase chromatography 5 chromatograms. The results are discussed in terms of possible post-transcriptional modifications of tRNA in the course of oogenesis. An alternative explanation resorting to changes in the tRNA population of the growing oocyte is also envisaged.