Clastogenic and aneuploidogenic effects of cigarette smoke condensate, mitomycin C and vincristine sulfate

1 January 1992

journal article
Published by Oxford University Press (OUP) in Mutagenesis

Vol. 7 (6) , 457-460
https://doi.org/10.1093/mutage/7.6.457

Abstract

Immunofluorescent staining of kinetochores in micronuclei (MN) of cytochalasin-B-blocked cells was used to distinguish between clastogenic and aneuploidogenic effects of cigarette smoke condensate (CSC), mitomycin C(MMC) and vincristine sulfate (VS) in Chinese hamster lung V79 cells by using an anti-kinetochore antibody. Within the base-line microncleated cells (20%), 50 to 58 % contained kinetochore positive (KC+) MN. MMC induced a significantly higher number of MN compared to the controls (P<0.01), and the majority of micornucleated cells (KC-) MN. In contrast, VS inudced a large number of MN (P<0.01) but most of the micronucleated cells contained KC+ MN (93% at 0.08 μ/ml). Cigarette smoke condensate (CSC) induced both KC+ (P<0.01) and KC-(P<0.05)MN suggesting the presence of both clastogenic and stronger aneuploidogenic agents. However, the higher frequency of KC+ than KC-micronucleated cells suggests a stronger aneuploidogenic effect of CSC.

Keywords

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