Antigenicity of fractions ofHelicobacter pylori prepared by fast protein liquid chromatography and urease captured by monoclonal antibodies
- 1 October 1990
- journal article
- conference paper
- Published by Springer Nature in European Journal of Clinical Microbiology & Infectious Diseases
- Vol. 9 (10) , 732-737
- https://doi.org/10.1007/bf02184685
Abstract
The antigenicity ofHelicobacter pylori protein fractions separated by fast protein liquid chromatography size exclusion was investigated by EIA with sera from patients of well definedHelicobacter pylori status. The antigenic material ofHelicobacter pylori was confined to fractions 8 and 14 to 21. Urease containing fractions (14/15) and flagella containing fractions (17/18) were identified. Fraction 8 non-specifically bound human immunoglobulin as demonstrated by the binding ofHelicobacter pylori negative sera. The remaining fractions 14 to 21 when used individually as EIA antigens were 91–100 % specific, however fractions 16 to 19 showed a reduced sensitivity (78 %) compared with the acid extract (95 %). The urease fractions were 91 % sensitive. Purified urease antigen captured by antiurease monoclonal antibodies was 83 % sensitive and 93.3 % specific.This publication has 19 references indexed in Scilit:
- Comparison of ELISA antigen preparations alone or in combination for serodiagnosing Helicobacter pylori infections.Journal of Clinical Pathology, 1990
- Antigenic conservation of the ureases of spiral- and helical-shaped bacteria colonising the stomachs of man and animalsFEMS Microbiology Letters, 1989
- Antigenic conservation of the ureases of spiral- and helical-shaped bacteria colonising the stomachs of man and animalsFEMS Microbiology Letters, 1989
- Comparison of different antigen preparations in an evaluation of the immune response toCampylobacter pyloriEuropean Journal of Clinical Microbiology & Infectious Diseases, 1988
- Immunoblot analysis of immune response to Campylobacter pylori and its clinical associations.Journal of Clinical Pathology, 1988
- Human serum antibody responses to the surface protein antigens of Campylobacter pyloridisSerodiagnosis and Immunotherapy in Infectious Disease, 1987
- Herpes simplex‐specific IgG subclass response in herpetic keratitisJournal of Medical Virology, 1987
- Identification of the Outer Membrane Proteins of Campylobacter pyloridis and Antigenic Cross-reactivity between C. pyloridis and C. jejuniMicrobiology, 1987
- Monoclonal antibodies directed against the flagella ofCampylobacter jejuni: production, characterization and lack of effect on the colonization of infant miceEpidemiology and Infection, 1986
- Variations in Surface Protein Composition Associated with Virulence Properties in Opacity Types of Neisseria gonorrhoeaeJournal of General Microbiology, 1979