Growth of protozoa in tissue culture 1. Plasmodium gallinaceum, exoerythrocytic forms

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Abstract
Cultivation of the exoerythrocytic forms of Plasmodium gallinaceum in tissue cultures of chicken cells of the macrophage reticulo-endothelial type is possible. Penicillin (10 units/ml.) does not stop the growth. An infection resembling a sporozoite infection can be obtained in chickens inoculated from such a culture. Erythrocytes can not be so infected. Sporozoites will not grow in such chicken cell cultures. Cultivation of P. gallinaceum by Traeger''s method for P. lophurae was not successful. The parasites lived longer, however, if glutathione was added, but would not multiply.