Complete 1H and 13C assignment of Lys and Leu sidechains of staphylococcal nuclease using HCCH‐COSY and HCCH‐TOCSY 3D NMR spectroscopy
- 9 April 1991
- journal article
- Published by Wiley in FEBS Letters
- Vol. 281 (1-2) , 33-38
- https://doi.org/10.1016/0014-5793(91)80352-4
Abstract
Complete proton and carbon sidechain assignments are reported for 22 lysine and II leucine residues in staphylococcal nuclease, an enzyme with 149 residues. These assignments are readily obtained in a direct manner from the correlations observed in the 3D HCCH‐COSY and HCCH‐TOCSY spectra and the unknown protein backbone assignments. These assignments open the way to detailed of the sidechain structure and dynamics at the active site, in the hydrophobic core and on the surface of the protein.Keywords
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