Genetic evidence for a product of the Fv-1 locus that transfers resistance to mouse leukemia viruses

Publisher Website
Google Scholar
Abstract
Extracts of mouse cells transfer resistance to N- or B-tropic host range types of mouse leukemia viruses. The genetic specificity of the inhibition was tested in 2 ways: by correlating the Fv-1 genotype of a number of mouse strains with the restriction-transferring activity of extracts of the respective embryo cell cultures, and by correlating the Fv-1 genotype of B6C3F2 (C57BL/6.female. [Fv-1bb] by C3H .male. [Fv-1nn] parental strains) mouse embryos, which segregate the Fv-1 alleles in a 1:2:1 ratio, with the inhibitor activity of extracts of the cells from each embryo. Five independent matings, totaling 45 individual embryos, were tested. Each embryo was cultured, and the Fv-1 genotype was determined independently by titration of N- and B-tropic viruses; the extracts of replicate secondary cultures were tested for their effect on infection of permissive cells by N- and B-tropic viruses. The specific-restriction-transferring activity of the embryos segregated with the appropriate Fv-1 genotype. Apparently the inhibitor of the leukemia virus host range types in the cellular extracts is a product of the Fv-1 locus.