Determination of Urinary 3-methoxy-4-hydroxymandelic Acid (Vanillylmandelic Acid) by Paper Electrophoresis

Abstract
A procedure is presented for the determination of 3-methoxy-4-hydroxymandelic acid (or vanillylmandelic acid) in urine. For separation from other phenolic acids, paper electrophoresis at low constant current is employed. Data are presented to show that this method is useful as a confirmatory test for pheochromocytoma, in which elevated urinary levels of vanillylmandelic acid are found.