The Iron-Hydrogen Peroxide-Iodide System Is Fungicidal: Activity Against the Yeast Phase of Blastomyces dermatitidis

Abstract
A series of experiments show the potency of a newly described microbicidal system, involving iron, H2O2, and halide, in killing a fungus (Blastomyces dermatitidis). B dermatitidis has previously been shown susceptible to the myeloperoxidase-H2O2 halide system. The present studies show killing of either of two strains in 1 hour if Fe+ + at 5 × 10−5M, H2O2 at 5 × 10M and Kl at 5 × 10−4M are all present (P < 0.001). EDTA, a Fe+ + chelator, abrogates killing. The mechanism presumably utilizes hydroxyl radical, since an inhibitor, ethanol, also neutralizes the system. The bactericidal and fungicidal system is of great potential importance in vivo.

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