Impairment of V(D)J Recombination in Double-Strand Break Repair Mutants
- 9 April 1993
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 260 (5105) , 207-210
- https://doi.org/10.1126/science.8469973
Abstract
Cells maintain the integrity of their genome through an intricate network of repair systems that recognize and remove lesions from DNA. The only known site-directed recombination process in vertebrates is the V(D)J recombination of lymphocyte antigen receptor genes. A large panel of cell lines deficient in DNA repair were tested for the ability to perform V(D)J recombination after introduction of the RAG-1 and RAG-2 genes. Two mutants failed to generate normal V(D)J recombination, and further analysis provided evidence for two distinct nonlymphoid-specific genes that encode factors involved in both DNA repair and V(D)J recombination.Keywords
This publication has 25 references indexed in Scilit:
- VDJ recombinationImmunology Today, 1992
- How many agrins does it take to make a synapse?Cell, 1992
- P transposition in Drosophila provides a new tool for analyzing postreplication repair and double-strand break repairMutation Research/DNA Repair, 1991
- Tn7 transposition in vitro proceeds through an excised transposon intermediate generated by staggered breaks in DNACell, 1991
- Coding joint formation of endogenous T cell receptor genes in lymphoid cells from scid mice: unusual P‐nucleotide additions in VJ‐coding jointsEuropean Journal of Immunology, 1991
- Kinetic and structural analysis of a cleaved donor intermediate and a strand transfer intermediate in Tn10 transpositionCell, 1991
- The scid mutation in mice causes a general defect in DNA repairNature, 1990
- RAG-1 and RAG-2, Adjacent Genes That Synergistically Activate V(D)J RecombinationScience, 1990
- Defective repair of DNA single- and double-strand breaks in the bleomycin- and X-ray-sensitive Chinese hamster ovary cell mutant, BLM-2Mutation Research/DNA Repair, 1989
- The defect in murine severe combined immune deficiency: Joining of signal sequences but not coding segments in V(D)J recombinationCell, 1988