Characterization of chromosomal and membrane associated plasmid in Bacillus brevis ATCC 9999.
- 1 January 1981
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 34 (3) , 323-330
- https://doi.org/10.7164/antibiotics.34.323
Abstract
A covalently closed circular(ccc)DNA, with MW of 44.7 .times. 106 daltons, was isolated from B. brevis ATCC 9999 (a gramicidin S producer) and from the gramicidin S-negative mutant EB16. The cccDNA in the case of the parent strain is mainly (99%) attached to the chromosomal and membrane fraction. A restriction enzyme map of the plasmid DNA was constructed for the enzymes SalI, SmaI and BamHI, which cleaved the plasmid DNA into 2, 2 and 6 fragments, respectively. Further digestion with the endonucleases EcoRI and HindIII cleaved the plasmid into 17 and 22 fragments.This publication has 8 references indexed in Scilit:
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