Ligand binding properties of the basophilic IgE receptor of normal and allergic individuals
- 1 March 1982
- journal article
- research article
- Published by Wiley in Clinical and Experimental Allergy
- Vol. 12 (2) , 121-129
- https://doi.org/10.1111/j.1365-2222.1982.tb01630.x
Abstract
The interaction of [125I]-human myeloma IgE with the human basophilic IgE receptor of normal and allergic individuals was quantitated by direct ligand binding analysis after removal of endogenous IgE. Using a mixed leukocyte preparation containing 2-5% basophils, the [125I]-IgE binding was specific for human IgE, paralleled the presence of basophils in the cell preparation, and was described by a time course similar to that reported for passive sensitization experiments. The Kd values were 4.0-13.2 nM for the normal individuals with 1.5-8.4 .times. 105 receptors per basophil, while the IgE receptor of allergic individuals was described by Kd values of 4.2-11.7 nM and 3.0-4.7 .times. 105 sites per basophil. Based upon the 11 individuals studied, the binding properties of the IgE receptor of normal and allergic individuals are apparently similar.This publication has 21 references indexed in Scilit:
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