Effect of CA++ and MG++ on Anticomplementary Sera in Complement-Fixation Tests

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Abstract
The action of normal mouse liver powder in removing anti-complementary (AC) properties from sera appears to be associated with Ca++ and Mg++. These ions eliminated AC activity from gamma globulin in a manner similar to that of mouse liver powder. Varying the amounts of Ca++ and Mg++ with individual human sera produced marked changes in their reactivity with complement. The introduction of Ca++ and Mg++ is less practical at present than the use of liver powder to eliminate AC properties.