Pheasant virus DNA polymerase is related to avian leukosis virus DNA polymerase at the active site
- 1 October 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 32 (1) , 78-90
- https://doi.org/10.1128/jvi.32.1.78-90.1979
Abstract
The DNA polymerase from Amherst pheasant virus (APV), a member of the pheasant virus species of retroviruses, was compared to the DNA polymerases of avian leukosis viruses (ALV) and a reticuloendotheliosis virus (spleen necrosis virus [SNV]). Immunoglobulin inhibition tests and competition immunoassays showed that APV and ALV DNA polymerases are closely related to their active sites. The determinants common to their active sites are not shared by SNV DNA polymerase. By using a species-specific radioimmunoassay, it was shown that APV and SNV DNA polymerases are grossly different from ALV DNA polymerase. The specificity of the relationship of the active sites of APV and ALV DNA polymerases was confirmed by a heterlogous radioimmunoassay. The data indicate that pheasant viruses are evolutionarily linked to ALV.This publication has 15 references indexed in Scilit:
- A competition immunoassay for characterizing the reverse transcriptase of mammalian RNA tumor virusesVirology, 1978
- Amino-terminal amino acid sequence of the major structural polypeptides of avian retroviruses: sequence homology between reticuloendotheliosis virus p30 and p30s of mammalian retroviruses.Proceedings of the National Academy of Sciences, 1978
- Radioimmunologic characterization of RD-114 reverse transcriptase: Evolutionary relatedness of mammalian type C viral pol gene productsVirology, 1978
- Efficient transcription of RNA into DNA by avian sarcoma virus polymeraseBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1976
- Pheasant virus: new class of ribodeoxyvirus.Proceedings of the National Academy of Sciences, 1976
- RNA tumor viruses of pheasants: Characterization of avian leukosis subgroups F and GVirology, 1974
- A rapid, sensitive, and specific method for the determination of protein in dilute solutionAnalytical Biochemistry, 1973
- A Single Subunit from Avian Myeloblastosis Virus with Both RNA-Directed DNA Polymerase and Ribonuclease H ActivityProceedings of the National Academy of Sciences, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- A Simple Chromatographic Method for Preparation of Gamma Globulin.Experimental Biology and Medicine, 1960