Detection of a luxS -Signaling Molecule in Bacillus anthracis
Open Access
- 1 July 2003
- journal article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 71 (7) , 3914-3919
- https://doi.org/10.1128/iai.71.7.3914-3919.2003
Abstract
Quorum-sensing regulation of density-dependent genes has been described for numerous bacterial species. The partially annotated genome sequence of Bacillus anthracis contains an open reading frame (BA5047) predicted to encode an ortholog of luxS , required for synthesis of the quorum-sensing signaling molecule autoinducer-2 (AI-2). To determine whether B. anthracis produces AI-2, the Vibrio harveyi luminescence bioassay was used. Cell-free conditioned media from vaccine (Sterne) strain 34F 2 induced luminescence in V. harveyi reporter strain BB170, indicating its production of AI-2. Cloned BA5047, expressed in Escherichia coli DH5α cells, restored AI-2 activity to these cells. To evaluate whether BA5047 is essential for AI-2 synthesis, it was deleted through allelic exchange with marker rescue; the resulting mutant had no functional luxS activity and had reduced growth in vitro. In the wild-type strain, AI-2 activity was greatest during the exponential phase of growth. In total, these data indicate that BA5047 is a functional luxS ortholog in B. anthracis necessary for growth-phase-specific AI-2 expression. Thus, B. anthracis may utilize extracellular signaling molecules to regulate density-dependent gene expression.Keywords
This publication has 23 references indexed in Scilit:
- Listening in on bacteria: acyl-homoserine lactone signallingNature Reviews Molecular Cell Biology, 2002
- Quorum sensing in Campylobacter jejuni: detection of a luxS encoded signalling moleculeMicrobiology, 2002
- Pseudomonas aeruginosa Quorum-Sensing Systems May Control Virulence Factor Expression in the Lungs of Patients with Cystic FibrosisInfection and Immunity, 2002
- Structural identification of a bacterial quorum-sensing signal containing boronNature, 2002
- Regulation of Gene Expression by Cell-to-Cell Communication: Acyl-Homoserine Lactone Quorum SensingAnnual Review of Genetics, 2001
- Crystal structure of the quorum-sensing protein LuxS reveals a catalytic metal siteProceedings of the National Academy of Sciences, 2001
- Intercellular Communication in Helicobacter pylori : luxS Is Essential for the Production of an Extracellular Signaling MoleculeInfection and Immunity, 2000
- Antibiotic-resistance cassettes for Bacillus subtilisGene, 1995
- Multiple signalling systems controlling expression of luminescence in Vibrio harveyi: sequence and function of genes encoding a second sensory pathwayMolecular Microbiology, 1994
- Methods for Computing Wagner TreesSystematic Zoology, 1970