Resistance to homologous and heterologous strains of potato virus Y in transgenic tobacco carrying the PVYN coat protein gene
Open Access
- 1 January 1997
- journal article
- Published by Canadian Science Publishing in Canadian Journal of Plant Science
- Vol. 77 (1) , 167-171
- https://doi.org/10.4141/p96-005
Abstract
Pure breeding lines of transgenic Nicotiana tabacum L. 'Delgold' were generated which expressed transcripts of the coat protein gene of the tobacco veinal necrosis stain of potato virus Y (PVYN) and were highly resistant to the virus. The form of resistance appeared to be RNA-mediated as no coat protein could be detected by enzyme linked immunosorbent assay. Resistant lines had two or three copies of the transgene but segregation analysis of the R1 and R2 progeny indicated that one copy in each line was functional for virus resistance. Simultaneous screening of lines for resistance against both PVYN and the heterologous PVYO (common) strain allowed for optimal selection of heterologous strain resistance. Resistance against the PVY-mn and PVY-nn strains and an ungrouped isolate, PVY-136, was less than against the PVYO isolate. The degree of heterologous strain resistance did not appear to directly correlate with the degree of sequence homology previously reported. The lines had no resistance against the related potato virus A and pepper mottle virus. Key words: Resistance to potato virus Y, transgenic, Nicotiana tabacum, enzyme linked immunosorbent assay, monoclonal antibodiesKeywords
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