GROWTH AND DIFFERENTIATION OF RAT BONE-MARROW STEM-CELLS IN DIFFUSION CHAMBER CULTURE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 5  (4) , 241-249
Abstract
The growth of granulopoietic progenitors (CFU-C) and morphologically identifiable hemopoietic cells from rat bone marrow was studied in intraperitoneal diffusion chambers over a 6-day period. After treatment of rats with repeated doses of hydroxyurea (HU), a bone marrow cell suspension was obtained wherein proliferating hemopoietic cells were reduced to a very low level and CFU-C to undetectable levels. During culture in diffusion chambers, growth of CFU-C, immature granulopoietic cells and macrophages was observed from this HU-treated bone marrow. In diffusion chamber culture stem cells preceding CFU-C are apparently able to rebuild functioning compartments of granulopoietic progenitors and morphologically identifiable hemopoietic cells.