Seasonal changes in the phytoplankton as indicated by chlorophyll estimations

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Abstract
The pigments of pure cultures of algae, of known number per volume, have been extracted with aqueous acetone after separation by filtration. The extracts were compared in a Kober colorimeter with standard solutions of a preparation of bought chlorophyll. The number required to give 1 mg. of chlorophyll was thus determined.The algae studied included: Chlorophyceae, two species ofChlamydomonasand oneChlorellanot yet described; Chrysophyceae,Dicrateria inornataParke; Bacillariophyceae,Coscinodiscus centralisEhr.,Thalassiosira gravidaCleve andNitzschia closterium(Ehr.) W.Sm. formaminutissima; Cryptophyceae,Hemiselmis rufescensParke; Dinophyceae,Gymnodiniumsp. The numbers per milligram of chlorophyll varied from 74 × 103forCoscinodiscusto over 3200 × 106forHemiselmis rufescens. Of the nine cultures, six were between 50 and 750 × 106. The volume of the cell is the decisive factor in the milligram count.

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