Some Properties of Porcine Carboxypeptidase N

Abstract
Some properties of carboxypeptidase N purified from pig serum were investigated. The amino acid composition resembles that of human carboxypeptidase N but differs markedly from that of porcine carboxypeptidase B [EC 3.4.17.2]. The enzyme contains a mass fraction of 0.1 carbohydrates. Both ester and peptide substrates are hydrolyzed at the same site. Peptide substrates are hydrolyzed if lysine or arginine is the C-terminal amino acid and provided that the penultimate amino acid is not proline. Argininic acid and .epsilon.-aminocaproic acid are good inhibitors of the enzyme.