Serum High-Density Lipoprotein: Effect of Change in Structure on Activity of Chicken Adipose Tissue Lipase
- 5 August 1966
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 153 (3736) , 640-641
- https://doi.org/10.1126/science.153.3736.640
Abstract
The high-density (1.063 to 1.21 g/ml) lipoprotein in human serum was analyzed as activator for a lipoprotein lipase isolated from chicken adipose tissue. The activating capacity was lost when the lipoprotein was extracted with a mixture of ethanol and ethyl ether (3:2 by volume) at —10°C and it was restored upon incubation of the extracted protein with aqueous sols of either whole phospholipids or the lecithin fraction prepared from the high-density lipoprotein. Since phospholipid sols alone proved ineffective as substrate activators, the complex which forms upon incubation of the extracted lipoprotein with phospholipids appears to be a necessary requirement for lipoprotein lipase activity.This publication has 12 references indexed in Scilit:
- The protein subunit of human serum lipoproteins of density 1. 125–1. 200 gram/mlBiochemical and Biophysical Research Communications, 1962
- The Fatty Acid and Positional Specificities of Lipoprotein LipaseJournal of Biological Chemistry, 1961
- Recombining Capacity toward Lipids of the Protein Moiety of Human Serum α1-LipoproteinJournal of Biological Chemistry, 1960
- Separation and characterization of the protein moiety of human α1-lipoproteinArchives of Biochemistry and Biophysics, 1958
- MODIFICATION OF HUMAN SERUM LIPOPROTEIN FRACTIONS BY LIPIDE EXTRACTIONJournal of Biological Chemistry, 1957