Kinetic Studies on Immune Hemolysis

Abstract
Summary: Ca++ is required in the formation of the complex EAC′x. The combination of EA with C′x is a reaction which essentially goes to completion. The velocity of formation of EAC′x is greater at 34°C than at 0°C. However, a more reactive EAC′x is obtained at 0°C. The reaction of EAC′x with C′y requires Mg++. The velocity and final extent of reaction of EAC′x with C′y, depends on the concentration of C′y, as well as on the concentration of C′x per cell.

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