TOXICITY OF SPUTUM DIGESTANTS TO TUBERCLE BACILLI IN WATER AND SPUTUM

Abstract
Prepn. of specimens suspect of containing tubercle bacilli for smear, culture and animal inoculation requires treatment with more or less toxic substances in order to free possible organisms from mucus and cellular debris, and to kill secondary organisms. The ideal substance would be one that is adequately digestant and lethal to all organisms but the tubercle bacillus. When the available agents for this purpose were tested for toxicity to tubercle bacilli, in aqueous suspensions and in homogenized sputum under practical diagnostic conditions, Na3PO4 and NaOH were found to be less toxic to the tubercle bacillus than HCl, H2SO4, oxalic acid and ammonium carbonate.
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