DNA sequence analysis of diuron‐resistant mutations in the mitochondrial cytochrome b gene of Saccharomyces cerevisiae
- 2 November 1986
- journal article
- Published by Wiley in FEBS Letters
- Vol. 208 (2) , 208-210
- https://doi.org/10.1016/0014-5793(86)81019-5
Abstract
Diuron (3-[34-dichlorophenyl]-1,1-dimethylurea), an inhibitor of mitochondrial respiration blocks the yeast respiratory chain between cytochrome b and c 1. Diuron-resistant mutants of Saccharomyces cerevisiae have been selected and several mutations localized to the mitochondrial cytochrome b gene. The present paper identifies specific DNA base changes within the cytochrome b gene conferring diuron-resistance. DNA sequence analysis was done utilizing primer extension of crude mitochondrial RNA preparations in the presence of reverse transcriptase. Five independent diuron-resistant mutations have been sequenced.Keywords
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