Effects of Shortened Erythropoietin Exposure on Sheep Marrow Cultures

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Abstract
To determine whether erythropoietin (Ep) is required only to trigger or also to sustain the growth of erythroid progenitors (CFU-E) in vitro, the effect of limiting the time of exposure of sheep bone marrow CFU-E to Ep on erythroid colony (EC) formation was examined. Restricting the in vitro exposure of CFU-E to 2-24 h, either by brief preincubation with Ep or by neutralization of Ep present in culture with anti-Ep, resulted in a 70-90% reduction of EC formation, and the colonies that were produced were small. No inhibition of EC size or number occurred when CFU-E were exposed to Ep for > 24 h. Ep may modulate the proliferative events subsequent to the initial triggering of CFU-E which leads to the formation of erythrocytes.