Analytical Studies on the Active Constituents in Crude Drugs. II. Determination of Berberine in Coptidis Rhizoma by High Performance Liquid Chromatography

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Abstract
A high performance liquid chromatographic method was developed for the determination of berberine in Coptidis rhizoma and Oriental pharmaceutical preparations. Retention time of berberine (flow rate: 1.0 ml/min) was approximately 4 min 40 s on a .mu.-Bondapak C18 colum (4 .times. 300 mm) with an eluent of acetonitrile:phosphate buffer (pH 5.2) = 60:40. Naphthalene was used as an internal standard, whose retention time was approximately 9 min 36 s. Berberine was extracted from the samples with methanol and injected into the column. Coefficient of variation for berberine content in Coptidis rhizoma was found to be less than 0.45%. It was concluded from the comparison of this method with 3 other methods (TLC, TLC densitometry and gas chromatography) that this method was significantly better than other methods with respect to simplicity, analytical time and accuracy.

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