Effect of physiological concentrations of insulin and antidiabetic drugs on RNA release from isolated liver nuclei

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Abstract
The addition of 10−11M insulin to a cell‐free system from rat liver promotes the release of messengerlike RNA from isolated prelabeled nuclei. The stimulation was similar whether the nuclei were preincubatcd with insulin, or if insulin was added directly to the cell‐free system with or without a protease inhibitor. Dot blot hybridization using cloned cDNA for α2u‐globulin mRNA showed that this was one of the messages whose release was enhanced by insulin. Nuclei isolated from rats treated with either of the antidiabetics tolbutamide or tolazamide showed no increase in RNA release in the presence of insulin over the concentration range 10−5−10−14 M. Furthermore, these nuclei did not release detectable levels of α2u‐globulin mRNA.