Feasibility Studies on Radiation Sterilization of Cephradine

Abstract
A comprehensive study was carried out to examine and evaluate the feasibility of radiation sterilization of cephradine antibiotic in the solid dry state. Absorbed gamma ray doses in the range of 5–25 kGy caused a dose-dependent loss of bactericidal activity. Moreover, radiation caused a dose-dependent degradation of the antibiotic and decrease in cephradine content due to radiation conversion to cephalexin. The former deleterious effect was detected spectrophotometrically and confirmed by high-performance liquid chromatography (HPLC). The latter effect was detected by NMR spectroscopy and by HPLC. The study revealed a noticeable change in the tint of the irradiated powdered drug which is dose-dependent and clearly remarkable at high radiation doses.

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