INCORPORATION OF RADIOACTIVE TRACERS OF SOME LABELLED COMPOUNDS INTO DNA, RNA, AND PROTEIN IN CHLORELLA CELLS AT VARIOUS STAGES OF THE CELL CYCLE IN SYNCHRONOUS CULTURE

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Abstract
Radioisotopically labeled compounds (14CO2, 32P-phosphates, [2-14C]acetate, L-[4,5-3H]leucine, [6-3H]thymidine, [2-14C]thymine, [5-3H]uridine and [2-14C]uracil) were fed for 1 h to synchronized Chlorella cells at various stages of the cell cycle. Uptake and incorporation of the tracers into DNA, RNA and protein of the algal cells were examined. They showed more or less characteristic patterns in the process studied. The 3 kinds of macromolecular cell constituents took up the radioactivities of all the compounds tested, except [6-3H]thymidine, whose radioactivity was incorporated into DNA and protein but not into RNA. 14C of CO2 seemed to be specifically or preferentially incorporated into chloroplast macromolecules under photosynthesizing conditions.