Identification of the adsorbing site of lysozyme onto the hydroxyapatite surface using hydrogen exchange and 1H NMR
- 8 February 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 317 (1-2) , 128-130
- https://doi.org/10.1016/0014-5793(93)81506-u
Abstract
The lysozyme-hydroxyapatite interaction was studied by measuring individual hydrogen-deuterium (H-D) exchange rates of amide protons. The H-D exchange reaction was initiated by transferring the lysozyme adsorbed on hydroxyapatite powder from H2O into D2O. After various H-D exchange time periods (pH 7.0, 25°C), the complex was dissociated and the remaining hydrogen label was determined by 2D NMR analysis. The H-D exchange rate of amide protons of residues 9, 11, 13, and 83 was slowed in the hydroxyapatite-lysozyme complex compared with free lysozyme. Residues 9, 11 and 13 positioned at the back of the active site would be the location of the binding site.Keywords
This publication has 9 references indexed in Scilit:
- Preparation and Properties of a Lysozyme Derivative in Which Two Domains Are Cross-Linked Intramolecularly between Trp62 and Asp1011The Journal of Biochemistry, 1991
- An Antibody Binding Site on Cytochrome C Defined by Hydrogen Exchange and Two-Dimensional NMRScience, 1990
- [26] Protein chromatography on hydroxyapatite columnsPublished by Elsevier ,1990
- Sequential proton NMR assignments and secondary structure of hen egg white lysozyme in solutionBiochemistry, 1988
- The Activity of Glucosyltransferase Adsorbed onto Saliva-coated HydroxyapatiteJournal of Dental Research, 1988
- Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filteringBiochemical and Biophysical Research Communications, 1983
- Amide proton exchange and surface conformation of the basic pancreatic trypsin inhibitor in solutionJournal of Molecular Biology, 1982
- Kinetics of the exchange of individual amide protons in the basic pancreatic trypsin inhibitorJournal of Molecular Biology, 1979
- Theory of chromatography on hydroxyapatite columns with small loadsJournal of Chromatography A, 1978