Large Scale Preparation of Liver Mitochondria with the Waring Blendor.
- 1 December 1953
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 84 (3) , 674-677
- https://doi.org/10.3181/00379727-84-20748
Abstract
A method is descr. for prepn. of mitochondria from 50 to 100 g. of liver. The tissue is pulped in a special tissue press and homogenized in isotonic sucrose at reduced motor speed for a short time in the Waring blendor. The mitochondria are isolated by differential centrifugation from isotonic sucrose. The ATP-ase, fatty acid oxidase, and succinoxidase activities of these prepns. were shown to be similar to those of mitochondria prepared with a pestle type homogenizer.Keywords
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