Transient co‐expression for fast and high‐yield production of antibodies with human‐like N‐glycans in plants
Open Access
- 8 May 2009
- journal article
- Published by Wiley in Plant Biotechnology Journal
- Vol. 7 (5) , 442-455
- https://doi.org/10.1111/j.1467-7652.2009.00414.x
Abstract
Plant-based transient expression is potentially the most rapid and cost-efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant-specific N-glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co-expression of an antibody, a suppressor of silencing and a chimaeric human β1,4-galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high-yield production of antibodies with human-like N-glycans and, more generally, provides solutions to many critical problems posed by the large-scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.Keywords
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