The relationship between antibody formation and deoxyribonucleic acid (DNA) synthesis in mouse spleen during primary and secondary response to sheep erythrocytes (SRC).

  • 1 August 1972
    • journal article
    • Vol. 23  (2) , 183-97
Abstract
Incorporation of [3H]thymidine into the spleens of intact mice during the primary and secondary responses to SRC has been studied. In both responses, auto-radiographs show a well marked increased incorporation of [3H]thymidine by cells in the peri-arteriolar zone during days 1 and 2. The degree of labelling indicated that many of these cells were in S phase in preparation for mitosis. A similar response occurred in cells situated in the mantle layer on day 3 to day 4 of the primary response, but was much less evident in the secondary response. This was in keeping with the results of scintillation counting which showed that increased uptake of [3H]thymidine was more sustained in the primary than in the secondary response to SRC.