Patterns of Amino-acid Inhibition of Alkaline Phosphatases as an Aid in Cancer Diagnosis

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Abstract
(1) The amino acid inhibition of alkaline phosphatases is a concentration-dependent, reversible process. (2) Isoenzymes from different organs and tumours have definite and reproducible patterns of amino acid inhibition in vitro. The use of 5 different amino acids (l-Phe, l-Arg, l-Homoarg, l-Met and l-Val) made it possible to establish such a pattern. (3) The enzymes in tumours are more sensitive to amino acid inhibition in vitro than those in their corresponding normal tissues. Different tumours show different amino acid inhibition patterns; there is no common tumoui type of alkaline phosphatase according to this criterion. (4) In the enzyme mixtures such as are found in human serum (unless one variant is present in an unusually high proportion) the individual components seems to influence mutually their respective characteristics in such a way that single variants cannot be distinguished, or their source traced with certainty.

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