Studies on vitamin E. 4. The simultaneous determinations of tocopherols, ubiquinones and ubichromenols (substance SC) in animal tissues: a reconsideration of the Keilin–Hartree heart preparation

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Abstract
A method is given for the simultaneous determinations of tocopherols, ubiquinones and ubichromenols in animal tissues. The substances are separated, after preliminary purification, on a 2-dimensional paper chromatographic system, from which they are eluted and separately measured. Vitamin A and tocopherylquinone can be determined in the same extract. The colorimetric determination of ubichromenol, with ferric chloride and [alpha][alpha][image] -dipyridyl, is described. The chromatographic behavior of ubiquinones and ubichromenols has been investigated and conditions are given for their separation. The existence of vitamin K1 in animal tissue has been confirmed. The Keilin-Hartree horse-heart preparation has been reinvestigated and earlier reports on the presence of [alpha]-tocopherol and [alpha]-tocopherylquinone have been confirmed. No loss of tocopherol, ubiquinone or ubichromenol was found after prolonged storage of tissues at -20[degree].