Fluorescent staining with bromocresol purple: A rapid method for determining yeast cell dead count developed as an assay of killer toxin activity
- 1 November 1993
- Vol. 9 (11) , 1207-1211
- https://doi.org/10.1002/yea.320091107
Abstract
A method is described for detecting yeast cells with plasma membrane damage, based on cell staining with bromocresol purple (BCP) which has a convenient fluorescence after entering the cells at pH below 5·2. The method was used to determine the activity of Saccharomyces cerevisiae pore‐forming killer toxin K1 in commonly used lethal units. The BCP test is rapid and as precise as the plating test.Keywords
This publication has 10 references indexed in Scilit: